Composite

Part:BBa_K3440005:Design

Designed by: Aditi Kallai, Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


Lux I with myc tag under Promoter prmA activated in the presence of PFOS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to show that LuxI can be expressed under prma promoter, and to compare expression with/without PFOS. This was used as our final construct in our E. coli module to detect PFOS. A moderate RBS (BBa_B0034) was used. A myc-tag was added for characterization.

Source

LuxI is from Aliivibrio fischeri (Vibrio fischeri) and pprmA is from Rhodococcus jostii (strain RHA1)

References